NanoEntek Cell Staining Solution AO/DAPI for EVE HT FL - 960 Tests

AO/DAPI Staining Solution is a single reagent composed of mixture of Acridine Orange and 4′,6-diamidino-2-phenylindole. This stain can be used to test cell viability with EVE™ HT FL. AO (Acridine Orange) and DAPI (4′, 6-diamidino-2-phenylindole) are fluorescent dyes that bind to DNA, staining the nucleus of targeted cells. AO is a permeable dye that stains the nucleus of all cells, regardless of the cell’s condition. DAPI is an impermeable dye that only stains the nucleus of non-viable cells with damaged membranes. To accurately evaluate the viability of cell types like PBMCs, stem cells, and splenocytes, it is advisable to use dual-fluorescence staining. This method effectively excludes non-nucleated cells (e.g., RBCs), platelets, and debris from the samples.