Moyen 199, modifié, avec des sels de oreilles, sans L-glutamine, bicarbonate de sodium, et rouge phénol, poudre, adapté à la culture cellulaire

Non disponible en dehors du Royaume-Uni et de l'Irlande

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Quantity

Formulated to contain 9.4 grams of powder per liter of medium.

Reconstitution

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.