Non disponible en dehors du Royaume-Uni et de l'Irlande
Analysis Note
The activity was determined by the dosedependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Biochem/physiol Actions
Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Packaging
10 µg in poly bottle
Physical form
Lyophilized from a 0.2 µm filtered solution of 1x PBS.
Preparation Note
HumanKine Interleukin-3 (IL-3), expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 17-45 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation which contributes to stability in cell growth media and other applications.
Ce produit répond aux critères suivants pour être admissible aux récompenses suivantes :