KITS TRUEGEL3D HYDROGEL

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

TRUE9-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic in-vivo conditions and to investigate the effect of matrix stiffness on cell physiology and behavior.SLO-PVA based gels are used to grow cells that do not need attachment to hydrogel for survival but rather use their secreted extracellular matrix. The SLO-PVA based hydrogels are preferred over dextran-based gels for long-term culture of cells producing carbohydrate degrading enzymes. TrueGel3DTM Hydrogel Kits has been used in the generation of 3D gel based tumor models.

Caution

Avoid prolong exposure of PEG non cell-degradable crosslinker to air and store SLO-PVA in small portions to avoid frequent freeze-thaw cycles.

Components

TRUE9-1KT contains SLO-PVA solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer and water.

Features and Benefits

Slow gelling (takes 6 minutes to 25 minutes, depends on gel stiffness) Flexibility in modulating gel stiffness Does not allow cell migration Does not allow cell recovery and post-culture analyses Cell inert hydrogel (if not customized with RGD peptide)

General description

The gel is formed by stable thioether bonds between thiol groups on PEG non cell-degradable crosslinker and thio-reactive groups on SLO-PVA. The polymer, SLO-PVA is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-PVA. SLO-PVA does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.