Pyrocarbonate de diéthyle, 97 %

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

Diethyl pyrocarbonate was used as:nuclease inhibitor for the extraction of undegraded nucleic acids from etiolated and green plant tissuesan antimicrobial additiveribonuclease inhibitor histidine residue modifierreagent for conversion of imines to carbamates

Biochem/physiol Actions

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

General description

Diethyl pyrocarbonate reacts selectively with the single-stranded loops of cruciform structures. The irreversible inactivation of ribonuclease with diethylpyrocarbonate has been studied.

Packaging

5 g in glass bottle

25, 100 g in poly bottle