LCAT Activity Assay Kit, Supplied by Roar Biomedical, Inc.

Not available outside of the UK & Ireland.

Analysis Note

Learn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.

Application

Applications for this method include high-throughput screening, mechanism of action studies and structure-activity relationship (SAR) work.

LCAT Activity Assay Kit has been used for measuring lecithin-cholesterol acyl transferase activity in plasma samples.

Features and Benefits

Compatible with high-throughput handling systems.

General description

The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol resulting in the formation cholesteryl ester. This enzymatic activity occurs on the surface of high density lipoprotein (HDL). The cholesteryl esters formed by LCAT may be packed into the core of HDL.

Principle

The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of (λ_Ex=390 nm/λ_Em=470 nm) LCAT. The assay may be validated by inhibition of LCAT with iodoacetate or another spectrally benign (not Ellman′s reagent) LCAT inhibitor.

Suitability

Suitable for measuring phospholipase activity of LCAT in plasma or serum.