Kit d'essai d'activité bêta-Lactamase, suffisant pour 100 tests colorimétriques

Non disponible en dehors du Royaume-Uni et de l'Irlande

General description

β-Lactamase (bL; EC 3.5.2.6) is an enzyme first identified in Escherichia coli and has been described as penicillinase. A number of βLs have since been identified from various bacteria. βLs specifically hydrolyze β-lactam rings present in antibiotics such as penicillin, cephalosporins, monobactam, and carbapenem, and confer resistance against these antibiotics.

Principle

The β-Lactamase Activity Assay Kit provides a simple assay for measuring βL activity in βL-secreting bacteria in fermentation media and bacterial cultures. It can also be used to detect βL-secreting bacteria in saliva, urine, and serum of infected mammals and in food samples. βL activity is measured by hydrolyzing a chromogenic cephalosporin called nitrocefin, producing a colorimetric product (A490), proportional to the enzymatic activity present. One unit of β-lactamase is the amount of enzyme required to hydrolyze 1.0 µmole of nitrocefin per minute at pH 7.0 at 25 °C.

Suitability

Suitable for the detection of β-lactamase activity in biological samples, food, and fermentation samples.