Kit MTP Activity Assay, fourni par Roar Biomedical, Inc.

Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Learn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.

Application

MTP Activity Assay Kit has been used to measure the activity of microsomal triglyceride transfer protein (MTP).

Features and Benefits

Compatible with high-throughput handling systems.

General description

Microsomal triglyceride transfer protein (MTP), a membrane-bound protein present in the liver, plays an important role in the assembly and secretion of very low density lipoprotein (VLDL). MTP is also necessary for assembly and secretion of chylomicrons by the intestine. This protein facilitates the transfer of triglyceride (TG), cholesteryl ester (CE) and phosphatidylcholine (PC) between phospholipid surfaces. Mutation in the MTP gene leads to abetalipoproteinemia.

Principle

The MTP Activity Assay Kit is a sensitive, homogeneous fluorometric assay useful for continuous measurement of MTP activity in cell lysate or tissue homogenate. This kit includes proprietary substrates that enable the detection of MTP-mediated transfer of neutral lipid. The transfer activity results in an increase in fluorescence intensity (λ_Ex=465 nm/λ_Em=535 nm).

Suitability

Suitable for measuring microsomal [triglyceride] transfer protein (MTP) activity in cell lysates or tissue homogenates, or for screening MTP activity inhibitors.