PEP Colorimetric/Fluorometric Assay Kit, sufficient for 100 colorimetric?�or?�fluorometric?�tests

Not available outside of the UK & Ireland.

Application

PEP Colorimetric/Fluorometric Assay Kit has been used to measure the concentrations of phosphoenolpyruvate.

Features and Benefits

Compatible with high-throughput handling systems.

General description

Phospho(enol)pyruvate (PEP) in an intermediate in carbohydrate metabolism involved in glycolysis and gluconeogeneis. In glycolysis, PEP is metabolized by pyruvate kinase to yield pyruvate. In plants, PEP is involved in the formation of aromatic amino acids as well as in the carbon fixation pathway.

Principle

PEP concentration is determined by a coupled enzyme assay, in which PEP is converted to ATP and pyruvate, resulting in a colorimetric (570 nm)/fluorometric (λ_ex = 535/λ_em = 587 nm) product, proportional to the PEP present.

Suitability

Suitable for the measurement of phosphoenolpyruvate (PEP) in tissue, cells and plant extracts