Fumarate Assay Kit, sufficient for 100 colorimetric?�tests

Application

Fumarate Assay Kit has been used to determine the concentration of fumarate in samples.

General description

Fumarate is a tricarboxylic acid (TCA) cycle intermediate formed by the oxidation of succinate by succinate dehydrogenase. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. Deficiencies in fumarate hydratase are associated with increased cellular concentrations of fumarate and increased incidences of renal cell cancer. This is most likely due to fumarate-mediated upregulation of HIF1. The Fumarate Assay Kit provides a convenient tool for sensitive detection of the fumarate in a variety of samples.

Principle

Fumarate is determined by an enzyme assay, which results in a colorimetric (450 nm) product, proportional to the fumarate present.

Suitability

Suitable for the detection of fumarate in a variety of samples including tissue, cells and serum