Phenylalanine Assay Kit, sufficient for 100 fluorometric?�tests

Not available outside of the UK & Ireland.

General description

Phenylalanine is a non-polar essential amino acid. In the liver, phenylalanine is converted to tyrosine, which is a precursor for multiple compounds including L-DOPA and melanin. Defects in the activity of phenylalanine hydroxylase result in the inherited metabolic disorder phenylketonuria (PKU). PKU results in the build up of phenylalanine and phenylalanine metabolites, and can result in growth defects and mental retardation if not treated.

Principle

Phenylalanine concentration is determined by a coupled enzyme assay, which results in the deamination of phenylalanine and the production of NADH which reacts with the probe resulting in a fluorescent (λ_ex = 535 nm/λ_em = 587 nm) product, proportional to the phenylalanine present.

Suitability

Suitable for L-phenylalanine detection in cell and tissue culture supernatants, serum, and other biological samples