Agar, testé pour la culture de cellules végétales, poudre

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Agar has been used as a componentof Gamborg′s B5 regeneration mediumof Murashige and skoog (MS) medium for the germination of Hypericum triquetrifolium Turra seeds in shoot inducing and naphthalene acetic acid pretreatment medium for the regeneration of Arabidopsis thaliana seedlings

Agar has been used as a component of EDMm (modified version of Eucalyptus dunnii basal medium (EDM)) for development of shoots of Eucalyptus dunnii clones. It has also been used as a component of Murashige and Skoog medium for in vitro culture of Strain TC09 ofCladosporium sphaerospermum.

Biochem/physiol Actions

Agar is majorly used as microbial solid culture media. It is used in the range of 0.5 to 1.0% (w/v) to solidify plant tissue culture media. It is essential for in vitro root development.

General description

Agar contains agarose and agaropectin, with agarose being the major gelling heteropolysaccharide. Agarose contains β-d-galactose and 3,6-anhydro-α-l-galactose, linked by glycosidic bonds β(1-4) called the neoagarobiose. It also has agarobiose with same disaccharide units with α(1-3) linkage. The major source of agar is the cell wall of seaweed from Rhodophyceae family.

Other Notes

Purified for research and protoplast culture.

Packaging

2.5 kg in poly drum

1 kg in poly bottle

100, 500 g in poly bottle

Preparation Note

Typical working concentration: 6-12 g/L.