HNE - DMA, hexane solution, > = 85 %(GC)

Non disponible en dehors du Royaume-Uni et de l'Irlande

Biochem/physiol Actions

Stable form of HNE; toxic second messenger of free radicals.

Packaging

2, 5 mg in glass bottle

Preparation Note

To prepare HNE from HNE-DMA: evaporate the hexane under a gentle stream of nitrogen at room temperature. Working at 4 °C, add 1 ml (5 mg size) or 0.4 ml (2 mg size) of 1 mM cold HCl and stir for 45 minutes, or alternatively, close the vial and shake for 30 minutes. Upon hydrolysis, the initially cloudy solution becomes almost clear. This solution now has a pH of ~3, contains ~5 mg (5 mg size) or ~2 mg (2 mg size) of the aldehyde (HNE), and is stable for at least 24 hours if kept at 4 °C. 6.8 mg HNE-DMA in 1 mL of hexane solution, giving 5 mg of HNE after hydrolysis.It is recommended that the freshly prepared HNE solution be used immediately.