MONOCLONAL ANTI-CD18 LOW ENDOTOXIN+

Not available outside of the UK & Ireland.

Application

The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 8 ug/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Biochem/physiol Actions

Cluster of differentiation (CD18) is involved in adhesion of leukocytes to the endothelium and its migration to the site of inflammation. Lower expression of CD18 is critical because of its involvement in all the heterodimeric complex and controls the expression of α subunit. CD18 is a part of intracellular signalling molecule, signals through phosphatidylinositol 3 kinase (PI3K) and tyrosine kinase. Mutations in ITGB2 causes autosomal recessive leukocyte adhesion deficiency type 1 disorder, characterized by frequent bacterial and fungal infections. Polymorphism in ITGB2 is associated with chronic lymphocytic leukemia (CLL). Mutations in CD18 causes Hirschsprung′s disease (HSCR)-associated enterocolitis (HAEC) and chronic colitis. ITGB2 is upregulated in atherosclerotic plaque.

Features and Benefits

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General description

ITGB2 (integrin β2) encodes common β-chain of the β2 integrin family, known as cluster of differentiation 18 (CD18). CD18 associates non-covalently with three different α subunits CD11a, CD11b, CD11c and forms three different heterodimers like lymphocyte function associated antigen-1 (LAD-1), macrophage antigen 1 (Mac-1), and p150,95 respectively. CD18 is primarily expressed in blood. The antibody MEM-148 recognizes an epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 (integrin beta2 subunit; beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. In human chromosome, the gene CD18 is localized on 21q22.3.

Immunogen

Peripheral blood mononuclear cells

Physical form

Solution in azide free phosphate buffered saline, pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.