Anticorps Anti- RUNX1 produit en anticorps isolé rabbit affinity

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Anti-RUNX1 antibody produced in rabbit is suitable for western blot at a working dilution of 1:500-1:1000.

Biochem/physiol Actions

RUNX1 (Runt-related transcription factor 1) plays a vital role in tumour suppression and oncogenic activities. In hematopoiesis, it is involed in hematopoietic development, hematopoietic stem cell homeostasis, and various blood malignancies. It may have clinicopathological impact on the proliferation of human bone marrow cells used in transplantation therapy.

Features and Benefits

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General description

The antibody detects endogenous level of total AML1(RUNX1) protein.

Runt-related transcription factor 1 (RUNX1) is a transcription factor that plays a vitral role in hematopoiesis, osteogenesis and neurogenesis. It is a member of Runt-related transcription factors (RUNXs). It was first identified as a component of Polyomavirus enhancer binding protein 2 (PEBP2) and Moloney murine leukemia virus enhancer core binding factor (CBF). Three isoforms of the protein have been identified: RUNX1a, RUNX1b and RUNX1c.

Immunogen

Peptide sequence around aa. 6-10 (D-A-S-T-S), according to the protein NP_001116079.1

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Target description

CBF binds to the core site, 5′-PYGPYGGT-3′, of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.