#-Amyloid Peptide (1-42), Hu 1PC X 0.25M

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Substrate Cleavage Assays (30-100 µg/ml)

General description

Wild-type, human Aβ_1-42 peptide. This product is supplied in a form that is not neurotoxic prior to a pre-incubation step. The level of toxicity has been shown to correlate to the extent of β sheet structure.

Wild-type, human Aβ_1-42 peptide. A number of mutations, identified in the gene encoding the β-amyloid precursor protein (βAPP), have been linked to early-onset Familial Alzheimer's Disease. Mutations in the genes encoding presenilin 1 and presenilin 2 have also been shown to alter the processing of βAPP, resulting in increased extracellular concentration of β-amyloid peptide Aβ1-42(43) relative to Aβ1-40. Biophysical and biochemical experiments suggest that Aβ1-42(43) may serve as a catalyst for the aggregation and deposition of β-amyloid peptide (Aβ) leading to neurotoxic effects associated with senile plaque formation. Furthermore, antibodies recog-nizing Aβ1-42 revealed that the long form of the peptide is increased in presenilin and βAPP mutants, while other studies have used Aβ specific antibodies to prevent the in vitro fibrillar aggregation of Aβ. Amino acid sequence verified by amino acid analysis or sequencing. This product is supplied in a form that is not neurotoxic prior to a preincubation step. The appearance of toxicity has recently been shown to correlate to the extent of β sheet structure. Useful for neurotoxicity studies and substrate cleavage assays.

Immunogen

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

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Citron, M., et al. 1997. Nature Med.3, 67.Hardy, J. 1997. Trends in Neurosci.20, 154.Solomon, B., et al. 1997. Proc. Natl. Acad. Sci. USA94, 4109.Duff, K., et al. 1996. Nature 383, 710.Iwatsubo, T., et al. 1994. Neuron13, 45.Suzuki, N., et al. 1994. Science264, 133.Simmons, L.K., et al. 1994. Mol. Pharmacol.45, 373.

Packaging

.25 mg in Glass bottle

Physical form

Supplied as a chloride salt.

Reconstitution

Reconstitute just prior to use. Do not store following reconstitution, as aggregation may occur.

Sequence

H-Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala-OH

Synthetic peptide corresponding to amino acid residues 1 - 42 of the processed human β-amyloid peptide.

Warning

Toxicity: Standard Handling (A)