Non disponible en dehors du Royaume-Uni et de l'Irlande
Application
Immunofluorescence microscopy of cells in culture (Bumoi et al., 1984; Schrappe et al., 1992). Also, Legg J et al (2003) Development 130:6049-6063 {3% PFA fixed frozen sections}.
Immunoelectron microscopy (Bumoi et al., 1984)
Immunoprecipitation: 1µg/10E6 cells (Bumoi et al., 1984; Morgan et al., 1981)
FACS analysis: 1-2µg/10E6 cells
Functional block of melanoma cell spreading (Morgan et al., 1981), glioma cell proliferation3 and tumor growth in nude mice (Morgan et al., 1981; Schrappe et al., 1992).
Optimal working dilutions must be determined by end user.
Detect Chondroitin Sulfate Proteoglycan using this Anti-Chondroitin Sulfate Proteoglycan Antibody, clone 9.2.27 validated for use in FC, IF & IP.
Research CategoryCell Structure
Research Sub CategoryECM Proteins
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose™ (Morgan et al., 1981).
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified immunoglobulin in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Specificity
Recognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250 kDa as well as precursor polypeptides of 210, 220 and 240 kDa (Bumoi et al., 1984). Reacts with human melanoma, glioma and proliferating brain endothelial cells.
Storage and Stability
Maintain between 2 and 8°C in undiluted aliquots for up to 6 months.
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