Anticorps Monoclonal Anti-Cdk4 produit en souris, clone DCS - 31, ascites fluid

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Monoclonal Anti-Cdk4 antibody was used in:immunoprecipitation of human melanoma cell line.immunofluorescence of fibrosarcoma cells.western blot analysis immunofluorescenceimmunohistochemistry

Biochem/physiol Actions

In association with cyclins, cyclin-dependent kinases (CDKs) forms active kinase complexes which is responsible for regulating cell cycle progression in eukaryotic cells. Within the complexes, the CDKs serves a catalytic protein kinase activity. This catalytic activity is regulated by two mechanisms: protein phosphorylation and association of regulatory subunits.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Cdk4 (mouse IgG2a isotype) is derived from the DCS-31 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cyclin-dependent kinase 4 is localized in human chromosome 12q14.1.

Cdk4 exists, in part, as a multi-protein complex with a D-type cyclin, proliferating cell nuclear antigen (PCNA) and a protein inhibitor, p21^Cip1. Cdk4 associates separately with p16, particularly in cells lacking a functional retinoblastoma protein.

Immunogen

recombinant human Cdk4 protein

Specificity

The antibody reacts specifically with Cdk4 and does not recognize other Cdk types.