Anticorps Anti - COMT produit dans le lapin, fraction IgG d' antisérum, solution aqueuse tamponnée

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Anti-COMT antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 5-10µL using human HepG2 cell lysates and western blotting at a dilution of 1:250-1:500 using whole extracts of mouse and rat liver.

Anti-COMT antibody produced in rabbit has been used in enzyme linked immunoassay (ELISA).

Biochem/physiol Actions

Catechol-O-methyltransferase (COMT) catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamine neurotransmitters including dopamine, noradrenaline, and adrenaline, their metabolites, and L-DOPA. Its major physiological role is to inactivate biologically active or toxic catechols. Variations in enzymatic activity of COMT result in the pathogenesis of different psychiatric and neurological diseases. COMT inhibitors are important therapeutic agents used in the treatment of Parkinson′s disease.

Catechol-O-methyltransferase (COMT) is referred as HEL-S-98n. The COMT Met/Met genotype individuals can acquire skill in executive function tasks (as multi-UV supervisory control to a higher level and faster than other genotype groups). The genetic variants of COMT alter neurobehavioral functions that are affected by mercury in adults and might modify the adverse neurobehavioral effects of mercury exposure in children.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Catechol-O-methyltransferase (COMT) is encoded by the gene mapped to human chromosome 22q11.21. It is widely expressed in neuronal and non-neuronal tissues. In mammals, two isoforms of COMT have been identified a cytosolic soluble form (S-COMT) and a membrane-bound form (MB-COMT), whose relative levels differ in various tissues and species.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The antibody recognizes MB-COMT and S-COMT.

Target description

COMT (Catechol-O-methyltransferase) catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative