Anti-Calbindin - D-28K (ex. : 20) anticorps produit en lapin, anticorps isolé affinité, solution aqueuse tamponnée

Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min at RT or 37 °C) improves immunohistochemical staining.

Application

Anti-Calbindin-D-28K (EG-20) antibody is suitable for use in immunohistochemistry (1:2,000 using sections of rat cerebellum), immunoblot and western blot (1:2,000 using a whole extract of rat brain). Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min., at RT or 37 ° C) improves immunohistochemical staining with the antibody.Anti-Calbindin-D-28K (EG-20) antibody has been used in immunohistochemical (IHC)peroxidase staining and cupric silver staining. It has also been used in immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Calbindin-D-28k is an intracellular protein characterized by EF-hand type structural motifs that bind to Ca²⁺. This protein modulates synaptic plasticity and signal transduction . Anti-Calbindin-D-28K (EG-20) antibody recognizes rat calbindin-D-28K (28 kDa). Due to interspecies similarity, reactivity with human, mouse, bovine, chicken, porcine, and frog is expected, but has not been confirmed. Staining of calbindin-D-28K is specifically inhibited with the calbindin-D-28K immunizing peptide by immunoblotting.

Calbindin-D-28K (CALB1) is a calcium binding protein in the brain. This 27kDa protein is located on human chromosome 8q21.

Immunogen

synthetic peptide corresponding to the C-terminal region of rat calbindin-D-28K (amino acids 225-244 with N-terminally added Lys-Gly) conjugated to KLH.

The immunogen sequence is specific for calbindin-D-28K and is not found in other members of the EF-hand family such as calbindin-D-9K, calretinin, myosin light chain, parvalbumin, S-100a, S-100b, S100A2 (S100L) and S100A6 (calcyclin). This sequence is highly conserved across species, including human, mouse, bovine, chicken, porcine, and frog calbindin-D-28K sequences.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.