Anti-ASXL2

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Anti-ASXL2, Cat. No. ABE1320, is a highly specific rabbit polyclonal antibody that targets Additional sex combs-like protein 2 and has been tested in Immunocytochemistry and Western Blotting.

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected ASXL2 in HeLa cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Putative Polycomb group protein ASXL2 (UniProt: Q76L83; also known as Additional sex combs-like protein 2) is encoded by the ASXL2 (also known as ASXH2, KIAA1685) gene (Gene ID: 55252) in human. ASXL2 is a putative Polycomb group (PcG) protein that acts by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. It is not required to initiate repression, but is essential to maintain it during later stages of development. They probably act via methylation of histones, rendering chromatin heritably changed in its expressibility. ASXL2 is shown to be involved in transcriptional regulation mediated by ligand-bound nuclear hormone receptors, such as peroxisome proliferator-activated receptor gamma (PPARG). It also acts as coactivator for PPARG and enhances its adipocyte differentiation-inducing activity; the function seems to involve differential recruitment of acetylated and methylated histone H3. ASXL2 is reported to be essential for haematopoiesis and acts as a haploinsufficient tumor suppressor in leukemia. ASXL2 gene is one of most commonly mutated genes in AML patients bearing the AML1-ETO fusion oncoprotein and loss of ASXL2 is shown to promote AML1-ETO-mediated leukemogenesis. ASXL2 protein contains two LXXLL motifs (aa 303-307 and 887-891), which are required for an association with nuclear receptors. It also contains an atypical PHD-type zinc finger regions (aa 1397-1434). Two isoforms of ASXL2 are reported that are produced by alternative splicing. Mutation in ASXL2 gene have also been linked to the development of Shashi-Pena syndrome that is characterized by delayed psychomotor development, intellectual disability, and macrocephaly, and neonatal feeding difficulties. (Ref.: Micol, JB et al. (2017). Nat. Commun. DOI: 10.1038/ncomms15429).

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the internal region of human Additional sex combs-like protein 2 (ASXL2).

Epitope: unknown

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Format: Purified

Quality

Evaluated by Western Blotting in Raji nuclear extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected ASXL2 in 10 µg of Raji nuclear extract.

Specificity

This rabbit polyclonal antibody detects Additional sex combs-like protein 2 (ASXL2) in human cells. It targets an epitope within 22 amino acids from the internal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~154 kDa observed; 153.82 kDa calculated. Uncharacterized bands may be observed in some lysate(s).