Anti-Misu / NSun2

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Research Sub CategoryRNA Metabolism & Binding Proteins

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Misu/NSun2 in human keratinocytes (Fry, M., and Watt, F. M. (2006). Curr Biol. 16(10):971-981.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Misu/NSun2 in human epidermis tissue (Fry, M., and Watt, F. M. (2006). Curr Biol. 16(10):971-981.).

Anti-Misu/NSun2 Antibody is an antibody against Misu/NSun2 for use in western blotting, ICC & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Misu/NSun2, also known as tRNA (cytosine(34)-C(5))-methyltransferase, Myc-induced SUN domain-containing protein (MISU), NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, tRNA (cytosine-5-)-methyltransferase, or tRNA methyltransferase 4 homolog (hTRM4), and encoded by the gene NSUN2/SAKI/TRM4, is a RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Misu/NSun2 methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Functionally Misu/NSun2 acts downstream of Myc to regulate epidermal cell growth and proliferation, and Misu/NSun2-mediated cytosine-5 methylation of vault noncoding RNA helps to determine the processing of them into regulatory small RNAs, and Misu/NSun2 also plays a critical role in gene expression. Interestingly too, Misu/NSun2 is required for proper spindle assembly and chromosome segregation, which is independent of its methyltransferase activity. Misu/NSun2 is localized in the nucleus and nucleolus during rest but during cytokinesis it can be localized in the spindle. Mutations are associated with mental retardation.

Immunogen

KLH-conjugated linear peptide corresponding to human Misu/NSun2.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Misu/NSun2 in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~90 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.