Anti-E3 ubiquitin - protéine ligase UHRF2

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Research Sub CategoryNuclear Receptors

Research CategoryEpigenetics & Nuclear Function

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected E3 ubiquitin-protein ligase UHRF2 in human testis and human kidney tissue.

Anti-E3 ubiquitin-protein ligase UHRF2 Antibody is an antibody against E3 ubiquitin-protein ligase UHRF2 for use in Immunohistochemistry and Immunocytochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

UHRF2 plays a critical role in cell cycle as it ubiquitinates cyclins and induces G1 arrest. UHRF2 also participates in epigenetic control of gene expression in many cells through binding and ubiquitinating DNA and histone binding proteins and targeting them for degradation. UHRF2 binds methylated CpG containing oligonucleotides as well and interacts directly with histone H3 as well as the trimethylated form of H3. UHRF2 is also recruited to areas of DNA damage and is important for DNA stability. UHRF2 is localized in the nucleus in heterochromatin. UHRF2 up regulated expression is associated with various cancers, however typically as mutated or lower copy number forms, which appear to has impaired anti-proliferation abilities.

Immunogen

KLH-conjugated linear peptide corresponding to the region required for interaction with histone H3 and interaction with PCNP of human E3 ubiquitin-protein ligase UHRF2.

Epitope: Region required for interaction with histone H3 and interaction with PCNP

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in WI-38 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected E3 ubiquitin-protein ligase UHRF2 in 10 µg of WI-38 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~58 kDa observed