AANAT (RT ); RBX- 50 µL

Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

ControlPOSITIVE CONTROL:

Night adapted rat pineal gland. Expression is light sensitive; highest levels are obtained from animals at night.

Application

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Western Blot: 1:10,000-1:20,000 using ECL. Reacts with the ~23 kDa AANAT protein. Some reactivity may also be seen at ~97 kDa. Suggested blocking buffer is PBS with 10% non fat dry milk and 0.2% Tween-20. Suggested dilution buffer is PBS with 1 mg/mL BSA fraction V. Suggested incubation time is overnight at room temperature (~23°C).

Optimal working dilutions must be determined by the end user.

Anti-AANAT Antibody is an antibody against AANAT for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide (phosphorylated) corresponding to amino acids near the N-terminous of rat AANAT conjugated to BSA. {Ganguly, S et al, 2005}.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Liquid rabbit serum containing no preservative.

Specificity

Recognizes AANAT (Arylalkylamine N-acetyltransferase). The antibody specifically recognizes the phosphorylated N-terminal PKA site of AANAT (pT31) (Ganguly et al. 2005). The antibody reacts with natural and recombinant protein.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.