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Analysis Note
ControlImmunohistochemistry Positive control: surrounding cartilage, neural tube / brainWestern blotting positive control: newborn mouse brain
Application
Immunohistochemistry (formalin fixed, paraffin embedded): 10 µg/mL. Tissues must be treated with Chondroitinase before staining {0.5U/mL in 100mM Tris-HCL pH 7.2-7.4, 30 minutes at room temperature} Then block and stain.
Western blotting: 0.5 µg/mL
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
This Anti-Versican Antibody, a.a. 535-598 of mouse versican is validated for use in IP, WB, IH(P) for the detection of Versican.
Research CategoryCell Structure
Research Sub CategoryECM Proteins
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan., Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest.
Immunogen
GST fusion protein containing amino acids 535-598 of mouse versican.
Epitope: a.a. 535-598 of mouse versican
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Immunoglobulin was purified by affinity chromatography and is presented in PBS with no preservative Anti-GST has not been adsorbed.
Specificity
Mouse versican GAG alpha domain
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Ce produit répond aux critères suivants pour être admissible aux récompenses suivantes :