Anti-IgG de la souris (molécule entière) - anticorps Phosphatase alcalin produit dans la chèvre, anticorps isolé affinity, solution aqueuse tamponnée

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Western blot analysis of nuclear or mitochondrial protein extracts were performed using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary antibody at a 1:5000 dilution in 5%BSA/TBS for 1 hour at room temperature. 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium (Sigma) was used for the substrate.

Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at µg/ml in TBS/Tween containing final concentration of 0.5M NaCl.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Affinity isolated antigen specific antibody is obtained from goat anti-mouse IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, that do not specifically bind to mouse IgG. Goat anti-Mouse IgG is conjugated to Sigma Alkaline Phosphatase, Type VII-S (Product No. P 5521) by protein crosslinking with 0.2% glutaraldehyde. Anti-Mouse IgG (Whole Molecule) is determined to be immunospecific for mouse IgG by immunoelectrophoresis (IEP), versus mouse IgG and normal mouse serum, prior to conjugation. Prior to conjugation, the antibody is found to be reactive with mouse IgG1, IgG2a, IgG2b, and IgG by Ouchterlony Double Diffusion (ODD). Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation.

Immunogen

Purified mouse IgG

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide.

Specificity

Binds all mouse Igs.

mouse IgG1, IgG2a, IgG2b, and IgG