Non disponible en dehors du Royaume-Uni et de l'Irlande
General description
Recombinant, Pneumocystis carinii RAD51 fused at the N-terminus to a His Tag™ sequence and expressed in E. coli. The Pneumocystis carinii RAD51 is equivalent to bacterial RecA and the homologue of yeast RAD51. It plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2.
Recombinant, Pneumocystis carinii RAD51 fused at the N-terminus to a His Tag™ sequence and expressed in E. coli. The Pneumocystis cariniiRAD51 is equivalent to bacterial RecA and the homolog of yeast RAD51. It plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2.
Legal Information
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
West, S.C. 2003. Nat. Rev. Mol. Cell Biol.4, 435.Jasin, M. 2002. Oncogene21, 8981.Symington, L.S., et al. 2002. Microbiol. Mol. Biol. Rev.66, 630.Daboussi, F., et al. 2002. Cell. Signal.14, 969.Liu, Y. and West, S.C. 2002. Breast Cancer Res.4, 9.
Packaging
10 ku in Plastic ampoule
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.
Unit Definition
One unit equals 1 ng of purified protein. 20 units are sufficient for a DNA-protein assay and 100 units are sufficient for a protein-protein interaction assay.
Warning
Toxicity: Standard Handling (A)
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