Non disponible en dehors du Royaume-Uni et de l'Irlande
General description
Full length, recombinant, human histone deacetylase 8 (HDAC8) fused to at the C-terminus to a His Tag™ sequence at and expressed in S. frugiperda insect cells using a baculovirus expression system. HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening.
Legal Information
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Waltregny, D., et al. 2005. FASEB J.19, 966.Buggy, J.J., et al. 2000. Biochem J. 350, 199
Packaging
50 µg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
Physical form
In 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN®-20 detergent, pH 8.0.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Unit Definition
One unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C.
Warning
Toxicity: Standard Handling (A)
Ce produit répond aux critères suivants pour être admissible aux récompenses suivantes :