Souris Anti-CD11a mAb (38)

Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Positive ControlMolt-4 cells

Application

Cell Inhibition Assays (1-5 µg/ml, see comments)r>Flow Cytometry (5 µg/ml)r>Frozen Sections (see comments)r>Immunoprecipitation (see comments)

General description

Protein A purified mouse monoclonal antibody. Recognizes the I domain of the ~180 kDa integrin α L subunit (CD11a).

Anti-CD11a, mouse monoclonal, clone 38, recognizes the ~180 kDa I domain of the αL integrin subunit in Molt-4 cells. It is validated for FC, IP, IHC on frozen sections and for cell inhibition assays.

Recognizes the ~180 kDa I domain of the α_L integrin subunit in Molt-4 human tumor cells.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Also blocks binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x10⁵ cultured Molt-4 human tumor cells using anti-CD11a antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. Variables associated with assay conditions will dictate the proper working dilution.

Holness, C.L., et al. 1995. J. Biol. Chem. 270, 877.Landis, R.C., et al. 1994. J. Cell. Biol. 126, 529.Dransfield, I., and Hogg, N. 1989. EMBO J. 8, 3759.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)