Non disponible en dehors du Royaume-Uni et de l'Irlande
Biochem/physiol Actions
Protein Target: WEE1
General description
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on ′Tyr-15′. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on ′Tyr-15′ and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Product Source: N-terminal GST-tagged and C-terminal 6His tagged, recombinant, human Wee1 amino acids 214-end expressed by baculovirus in Sf21 insect cells.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Packaging
Also available in 250µg size as catalog # 14-925M and in bulk as catalog # 14-925-K.
Quality
Routinely evaluated by phosphorylation of peptide LSNLYHQGKFLQTFCGSPLYRRR.
Ce produit répond aux critères suivants pour être admissible aux récompenses suivantes :