Non disponible en dehors du Royaume-Uni et de l'Irlande
Analysis Note
Specific Activity: This lot was successfully phosphorylated in a non-radioactive kinase assay using recombinant active MAP Kinase 2/Erk2 (Catalog # 14-173). MBP phosphorylation was detected by immunoblot analysis using anti-phospho-MBP (Catalog # 05-429). Immunoblot shown to the right.
General description
Product Source: Recombinant fusion protein containing bovine myelin basic protein (MBP) with an N-terminal GST tag and a C-terminal 6His tag, expressed in E. coli.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Purity information is lot specific, please consult the certificate of analysis
Physical form
500 µg recombinant MBP in 500 µl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 500 mM imidazole, 1 mM PMSF, and 10% glycerol. Frozen solution.
ProBond Resin chromatography
Quality
Routinely evaluated by kinase assay
Storage and Stability
Stable for 2 years at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Ce produit répond aux critères suivants pour être admissible aux récompenses suivantes :