Analysis Note
www.gelifesciences.com.
Application
HiTrap® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System
Features and Benefits
• For optimizing purification of histidine-tagged Proteins using different kinds of metal ions.• Conveniently charge with your metal of choice.• Iminidoacetic acid coupled to Sepharose® High Performance.• Purifying Proteins with exposed histidine groups via metal ion complex formation.• Simple operation with a syringe, pump or chromatographic system such as ÄKTA® design or FPLC™ System.• Packed with Chelating Sepharose® High Performance• Simple operation with a syringe, pump or chromatographic system such as ÄKTATMdesign or FPLCTM System
General description
HiTrap® Chelating HP is prepacked with Chelating Sepharose® High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.
Legal Information
HiTrap is a registered trademark of GE Healthcare
ÄKTA is a registered trademark of GE Healthcare
Sepharose is a registered trademark of GE Healthcare
FPLC is a trademark of GE Healthcare
Storage and Stability
Store at 4 to 30 °C (20% Ethanol)
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