Alginate Lyase, powder, >=10,000 units/g solid

Not available outside of the UK & Ireland.

Application

Alginate Lyase has been used: in the pre-processing of enzyme to de-encapsulate cellsto digest Pseudomonasexopolysaccharide to facilitate electroporation

Alginate Lyase is used to break down alginate or alginic acid and to reduce viscosity. It is useful during follicle isolation, encapsulation, and culture .

Biochem/physiol Actions

Breaks down alginate or alginic acid to smaller molecules and reduces viscosity.

Alginate lyases actively participates in the saccharification of the acidic polysaccharides for the production of bioethanol.

General description

Alginate lyases is present in several marine algae, marine mollusks and a vast range of marine and terrestrial bacteria.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

100 mg in poly bottle

Preparation Note

Add 0.15 mI of enzyme solution (1 un/ml) to 4.5 ml of 0.1% sodium alginate (pH 6.3). Incubate at 37 °C for 30 minutes. Terminate reaction by addition of 4.65 ml of 0.1 N NaOH.

Unit Definition

One unit will produce an increase the A_235nm of 1.0 per minute per mL of sodium alginate solution at pH 6.3 at 37 °C