TAMPON TBE 10 x PH 8,3 TRIS-BORATE - EDTA

Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Appearance (colour): almost colourlessAppearance (description): almost clearpH-value: 8.2 - 8.4

General description

Tris, borate, and ethylenediaminetetraacetic (TBE) buffer is routinely used to conduct DNA and RNA agarose gel electrophoresis. It comprises a weak acid in neutral and anionic forms (e.g., COOH and COO^-species) and a weak base, Tris, which is either in neutral or cationic forms (Tris-NH₂and Tris-NH₃⁺). These ions buffer the pH, maintain a low conductivity medium, and transfer the electrical current. Ethylenediaminetetraacetic (EDTA) is used to chelate Mg²⁺ ions and inactivate the potential DNA nucleases. TBE is also used to perform a good resolution of DNA fragments that slightly enhances the separation of smaller fragments. It also protects nucleic acids from enzymatic degradation.

Packaging

2.5 l in Plastic bottle