CELLULES MCF10A ESR2 (-/ -),

Application

The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°

Cell freezing medium-DMSO 1X, Cat. No. C6164

Media renewal changes two to three times per week.

Features and Benefits

Zinc finger nuclease (ZFN) knockout on chromosome 14q23.2

These MCF10A cells are adherent with a doubling time of approx. 20 hours

General description

This cell line corresponds to ATCC Catalog No. CRL-10317.

MCF10A CELLS ESR2 (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.