TSKgel Reversed Phase HPLC Column (Polymer-based), phase C18-NPR, L x I.D. 3.5 cm x 4.6 mm, 2.5 mum particle size

Not available outside of the UK & Ireland.

General description

Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-NPR provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. NPR, non-porous resin, columns are prepared from non-porous methacrylate particles of uniform 2.5 micron size, which provides high efficiency separations and fast anaylses of peptides and proteins. The non-porous particle structure limits product isolation to sub-microgram loads.

Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns. Octadecyl-2PW particles have 125Å pores Octadecyl-4PW particles have 500Å pores Phenyl-5PW RP particles have 1000Å pores Octadecyl-NPR particles are nonporous

Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Physical form

Shipped in 70% methanol - 30% waters (v/v).

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