Insémination Atom ilomycin A1, > 1PCX15UG

Non disponible en dehors du Royaume-Uni et de l'Irlande

Biochem/physiol Actions

Primary Targetvacuolar-type H⁺-ATPase

Cell permeable: yes

General description

A macrolide antibiotic that acts as a specific inhibitor of vacuolar-type H⁺-ATPase (V-type; K_i = 0.5 nM) and is a valuable tool for distinguishing among different types of ATPases. Reported to selectivity inhibit β-secretase, an enzyme involved in the processing of amyloid precursor protein (APP). Blocks lysosomal cholesterol trafficking in macrophages. Known to interfere with pH regulation in brain cells. Inhibits bone resorption both in vivo and in vitro. Has been shown to exhibit cytotoxic effects on a number of cell lines in a cell viability assay.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sinha, S., and Lieberburg, I. 1999. Proc. Natl. Acad. Sci. USA96, 11049.Calvert, C.M., and Sanders, D. 1995. J. Biol. Chem.270, 7272.Nishihara, T., et al. 1995. Biochem. Biophys. Res. Commun.269, 255.Crider, B.P., et al. 1994. J. Biol. Chem.269, 17379.Palokangas, H., et al. 1994. J. Biol. Chem.269, 17577.Sundquist, K.T., and Marks, S.C., Jr. 1994. J. Bone Miner. Res.9, 1575.Drose, S., et al. 1993. Biochemistry32, 3902.Furuchi, T., et al. 1993. J. Biol. Chem.268, 27345.Bowman, E.J., et al. 1988. Proc. Natl. Acad. Sci. USA85, 7972.

Packaging

Packaged under inert gas

15 µg in Glass bottle

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C. Use only fresh DMSO for further dilutions.

Warning

Toxicity: Standard Handling (A)