Instigateur Aphidicolin

Non disponible en dehors du Royaume-Uni et de l'Irlande

Biochem/physiol Actions

Reversible: yes

Primary TargetDNA polymerase α and δ

Cell permeable: yes

General description

A cell-permeable tetracyclic diterpene antibiotic. Cell synchronization agent. Blocks the cell cycle at the early S-phase. Specific, non-competitive and reversible inhibitor of DNA polymerase α and δ in eukaryotic cells and in some viruses of animal origin. Potentiates apoptosis induced by arabinosyl nucleosides in leukemia cell lines. Also induces apoptosis in HeLaS3 cells, but inhibits vincristine (>Cat. No. 677181)-induced apoptosis in the p53-negative human prostate cancer cell line PC-3.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Borner, M.M., et al. 1995. Cancer Res.55, 2122.Poluha, W., et al. 1995. Oncogene10, 185.Urbani, L., et al. 1995. Exp. Cell Res.219, 159.Schimke, R.T., et al. 1994. Philos. Trans. R. Soc. London. B. Biol. Sci.345, 311.Kuwakado, K., et al. 1993. Biochem. Pharmacol.46, 1909.Hubermann, J.A. 1981. Cell23, 647.

Packaging

Packaged under inert gas

1 mg in Glass bottle

Physical form

A 30 mM (1 mg/98 µL) sterile-filtered solution of Aphidicolin (Cat. No. 178273) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C. Use only fresh DMSO for further dilutions.

Warning

Toxicity: Irritant (B)