OZ Biosciences ExploCap GFP saRNA Modified with 5-methyl-Cytidine (m5C)

ExploCap SaGFP RNAs have been designed to produce high expression level of GFP protein with enhanced length of duration. OZB RNAs are produced by in vitro transcription and are co-transcriptionally capped using trinucleotides ExploCap analog and contain a poly(A) tail at the 3’ end. Sequences have been optimized to yield improved stability and performance.

OZB saGFP RNA has been constructed based on replicons of positive-sense ((+)-RNA) viruses (Venezuelan equine encephalitis Virus (VEEV)), where the coding sequence of the viral structural proteins is replaced with that of a gene of interest (GOI, here GFP), while retaining the coding sequences of non-structural proteins (NsPs), including the viral RNA-dependent RNA polymerase. Please note that OZB saRNAs encodes also for Puromycin Resistance Cassette, which does not alter the expression of the GOI but can be used for cell selection.

ExploCap GFP saRNAs:

#MRNA119 does not bear any additional nucleotide modifications.

#MRNA120 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine).

• Cap Modification: ExploCap™
• Poly (A) Tail: Yes
• Concentration: 1.0 mg/mL
• Buffer: 1 mM Sodium Citrate, pH 6.4
• Full length mRNA: 9821 nt
• Size: 20 µg, 100µg and 1 mg
• Storage: -80°C
• Shipping: Dry ice (fee included in shipping cost)