Meridian Bioscience Lyo-Ready Genotyping Direct qPCR Stool 4x - 200 Reactions (1mL)

Lyo-Ready Genotyping Direct qPCR Stool has been optimised to provide highly specific allelic discrimination as demonstrated by excellent cluster separation, even in the presence of PCR inhibitors found in crude human stool samples. Furthermore, it can be used in a liquid or lyophilised format to create ambient-temperature stable assays, making it ideal for point-of-care (POC) devices.

SNPs (single nucleotide polymorphisms) or point mutations are the most common types of genetic variation and comprise the major part of phenotype diversity between individuals. SNPs can be responsible for both resistance or susceptibility to a certain disease and once individual SNPs have been identified by sequencing, they can be used for pharmacogenetics, in evaluating and predicting a patient’s response to treatment and risk of adverse events, or for diagnostics, such as in cancer screening. Clinical screening of SNPs requires large-scale multiplexed genotyping, which traditionally relies on DNA extraction from complex samples such as blood, to achieve sensitive DNA amplification. However, blood collection is invasive and requires a professional, stool on the other hand is non-invasive, safe, less expensive and easy to do at home, making it ideal for POC and frequent sampling.

Meridian’s new Lyo-Ready Genotyping Direct qPCR Stool is a mix that is designed for fast, precise, and reproducible allelic discrimination and cluster separation. Its advanced formulation enables highly sensitive amplification of SNPs and other point mutations from stool samples and overcomes inhibitors such as bile salts that can damage DNA and inhibit PCR reactions. Furthermore, it can be used in a wet format or lyophilised to create ambient-temperature stable assays, making it ideal for point-of-care (POC) devices.

• Ultra-sensitive detection down to less than 1 copy using direct amplification protocols
• Inhibitor-tolerant to PCR inhibitors found in stool
• Tight fluorescence clusters with clear allele discrimination, perfect for difficult SNPs
• Can be used as a liquid or lyophilised, ideal for high-throughput or POC testing