This is a medium for the selective isolation of streptococci, particularly Streptococcus agalactiae, involved in bovine mastitis. The medium is enriched by the addition of 7% sheep blood and made selective by the inclusion of crystal violet and thallous sulphate. Aesculin is also present and assists in the differentiation of Streptococcus agalactiae, which give rise to blue colonies, from aesculin positive group D streptococci which produce black colonies.
- Storage temperature: 2 to 8°C
- Appearance: firm gel
- Colour: dark red
- Recommended incubation: aerobically at 37°C for 16 to 24 hours
- pH: 7.3 ± 0.2
- Plate size: 90mm
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