NIPPON Genetics EUROPE Electrophoresis Starter Pack II (MIDORIGreen Direct + 100bp DNA Marker + 100g Agarose + Agarose Gel Band Cutter)

Electrophoresis Starter Pack II includes:

MIDORI^Green Direct DNA/RNA Stain 1mL: a safe alternative to ethidium bromide for visualisation of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. In contrast to most other non-ethidium bromide based dyes, MIDORI^Green Direct is just added to your samples. The loading dye is already included. You do not need to add any other loading dye to both gel matrix and running buffers. The direct staining of the DNA rather than the gel eliminates the background staining providing a perfect signal. MIDORI^Green Direct was developed to work with blue light LED illuminators, but you can also use it with a regular UV transilluminator. The isolation of DNA from agarose gels enables downstream applications. It is well known that many dyes, such as ethidium bromide or even SYBR™ Green are strong enzyme inhibitors due to their intercalating properties. MIDORI^Green dyes bind to the DNA backbone. This results in a much higher efficiency for downstream applications, i.e. cloning, sequencing, PCR, etc.

FastGene® MWD100 100bp DNA Marker 50ug/500uL: the perfect ladder for everyday use; the ladder with 12 fragments starts at 100bp, therefore being suitable even for small qPCR products, and goes up to 3000bp so that the sizes of small plasmids and big PCR products can be determined.

FastGene® Agarose 100g: developed for a rapid separation of DNA fragments, such as PCR products and plasmid DNA, as well as RNA fragments. The very high quality was graded as suitable for molecular biology. The purity of the agarose leads to an excellent transparency and a low background. This is especially important to obtain sharp and well defined DNA and/or RNA bands with the highest sensitivity in the low molecular weight range. The high gel strength allows to use concentrations from 0.75 to 2%. The lack of DNases and RNases is assayed as well as the background fluorescence with ethidium bromide is determined.

FastGene® Agarose Gel Band Cutter sample: this easy-to-use tool will facilitate your daily work. Now you can excise your DNA bands easily without risking contamination or scratching the glass surface of the transilluminator. The FastGene® Agarose Gel Band Cutter is a ready-to-use and disposable tool for cutting agarose gel bands. This affordable tool simplifies fragment purification and eliminates wasted effort using razor blades. The size of the excised gel band will always be 6 x 3mm, and you can collect multiple bands in a single FastGene® Cutter, making large-scale purifications that much easier.