Defined Bioscience’s HiDef® B8 is a complete, serum-free, defined formulation designed for the feeder-free maintenance and expansion of human pluripotent stem cells (PSCs) in an undifferentiated state.
To better support small-scale experiments and pilot studies, the HiDef® B8 400x supplement is offered in two vial sizes. Standard vials prepare 500mL of complete medium, while miniature vials prepare 50mL, enabling convenient preparation of smaller volumes without excess waste.
HiDef® B8 contains insulin, ascorbic acid-2-phosphate, transferrin, sodium selenite, TGF-β3, NRG-1, and thermostable FGF-2/bFGF. The addition of further components is not required.
- Application: Induced pluripotent stem cell culture and maintenance
- Fully defined
- Convenient use
- Weekend-free
- Tested across >100 hiPSC and hESC lines
- Animal origin free (AOF)
- Store at -20˚C, protect from light
- Product supplied in liquid form
This product has met the following criteria to qualify for the following awards: