MPO FLUOROMETRIC ACTIVITY ASSAY KIT

Not available outside of the UK & Ireland.

Application

MPO Fluorometric Activity Assay Kit has been used to determine the activity of myeloperoxidase.

General description

Myeloperoxidase (MPO) is a heme-containing enzyme that catalyzes the hydrogen peroxidase-mediated oxidation of halide ions to hypohalous acid. MPO is a lysosomal protein, highly expressed in neutrophils, that plays a role in the antimicrobial actions that occur as a result of neutrophil stimulation. The actions of MPO may also contribute to the initiation and pathogenesis of cardiovascular disease.

Principle

The MPO Activity Flurometric Assay kit provides a simple and direct procedure for measuring MPO activity in a variety of samples. In this assay, MPO catalyzes the formation of hypochlorous acid with reacts with the substrate, aminophenyl fluorescein, to generate fluorescein) (λ_ex = 485/λ_em = 525 nm). One unit of MPO is the amount of enzyme that will oxidize the MPO substrate to yield 1.0 µmole of fluorescein per minute at room temperature.

Suitability

Suitable for the measurement of myeloperoxidase (MPO) in biological samples such as tissue, cells, serum and white blood cells