Trizma acetate, BioUltra, >=99.0% (NT)

Code: 93337-100g D2-231

Not available outside of the UK & Ireland.

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for...


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$378.28 100G
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Not available outside of the UK & Ireland.

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma acetate is used to make Tris acetic acid buffers that are used as diluents for various assays and as an electrophoresis running buffers.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

Recommended buffer for maximum sensitivity of ATP assays with firefly luciferase; Assay of glutamate binding

λ0.5 M in H2O
anion tracessulfate (SO42-): ≤50 mg/kg, chloride (Cl-): ≤50 mg/kg
assay≥99.0% (NT)
cation tracesBi: ≤5 mg/kg, Na: ≤50 mg/kg, Mo: ≤5 mg/kg, Al: ≤5 mg/kg, K: ≤50 mg/kg, As: ≤0.1 mg/kg, Li: ≤5 mg/kg, Cr: ≤5 mg/kg, Mn: ≤5 mg/kg, Sr: ≤5 mg/kg, Mg: ≤5 mg/kg, Ca: ≤10 mg/kg, Pb: ≤5 mg/kg, Cu: ≤5 mg/kg, Ba: ≤5 mg/kg, Fe: ≤5 mg/kg, Zn: ≤5 mg/kg, Ni: ≤5 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg
formcrystalline powder
ign. residue≤0.2%
impuritiesinsoluble matter, passes filter test
InChI keyPIEPQKCYPFFYMG-UHFFFAOYSA-N
InChI1S/C4H11NO3.C2H4O2/c5-4(1-6,2-7)3-8;1-2(3)4/h6-8H,1-3,5H2;1H3,(H,3,4)
loss≤0.2% loss on drying, 20 °C (HV)
pH6.0-7.0 (25 °C, 0.5 M in H2O)
product lineBioUltra
Quality Level300
SMILES stringCC(O)=O.NC(CO)(CO)CO
solubilityH2O: 0.5 M at 20 °C, clear, colorless
UV absorptionλ: 280 nm Amax: 0.05, λ: 260 nm Amax: 0.06
Cas Number6850-28-8
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