cAMP Biotrak(TM) EIA; (Non-Acetylation Protocol; Lysis Reagents not Included); Cytiva; RPN2251

Code: gerpn2251 D2-232

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Application

cAMP Biotrak

read more

Your Price
$779.70 EACH
Discontinued
This product has been discontinued. Please click here for alternatives

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Application

cAMP Biotrak EIA, (Non-Acetylation Protocol) has been used to measure intracellular cyclic adenosine monophosphate (cAMP) levels.

Features and Benefits

• Elimination of inconvenient, time-consuming extractionprocedures.• Flexible method-choice of assay protocols.• Range 25 - 6400 fmol/well (non-acetylation protocol).• Rapid assay protocol.• Nonradioactive.• Specific for cAMP.• Precise and accurate measurement• Ready-to-use substrate.• Colour coded reagents

General description

EIAs and ELISAs are convenient, high-sensitivity, peroxidase-based technologies that enable the amount of protein present in a sample to be measured in a single day. Novel lysis reagents have been developed (Lysis Reagents 1 and 2) to facilitate simple and rapid extraction of cellular molecules.The Biotrak cAMP competitive enzymeimmunoassay system is specifically designed for research purposes. The kit includes protocols using novel lysis reagents in order to facilitate simple and rapid extraction of cAMP from cell cultures. These components avoid the requirement for traditional, time-consuming extraction procedures and obviate the need for removal of extraction reagents prior to measurement. It combines the use of a peroxidase-labeled cAMP conjugate, a specific antiserum which can be immobilized on to precoated microplates, and a one-pot stabilized substrate solution.Lysis reagent 1 hydrolyzes cell membranes to release intra-cellularcAMP. Lysis Reagent 2 sequesters the key component in LysisReagent 1 and ensures cAMP is free for subsequent analysis. Thedetergent/sequestrant complex does not interfere with antigen:antibody binding. Lysis Reagent 1 is simply added to cultured cells,followed by a 5 to 10 min incubation before assay. Theantiserum is reconstituted with Lysis Reagent 2. The assay is basedon competition between unlabeled cAMP and a fixed quantity ofperoxidase-labeled cAMP, for a limited number of binding sites on acAMP specific antibody.

Legal Information

Biotrak is a trademark of GE Healthcare

shelf lifePlease be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
mfr. no.GE Healthcare, RPN2251
packagingpkg of 96 wells
storage temp.2-8°C
This product has met the following criteria to qualify for the following awards:



ALTERNATIVE PRODUCTS

PROCEED TO CHECKOUT

HAVE AN ACCOUNT? LOGIN


GUEST CHECKOUT

Proceed as a guest. You will have the option to register to access exclusive pricing and stock availability features after checkout.