Anti-Gapex-5 antibody produced in rabbit, approx. 1.0 mg/mL, affinity isolated antibody

Not available outside of the UK & Ireland.

Application

Anti-Gapex-5 antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Rab5-activating protein 6 (RAP6) or Gapex-5 is an evolutionary conserved protein and its C-terminal Vps9 domain is responsible for the interaction with guanosine diphosphate GDP bound Rab5 and an N-terminal Ras GTPase-accelerating proteins (GAP) domain. Gapex-5 has guanine nucleotide exchange factor (GEF) activity specific for Rab5 and GAP activity specific for Ras. It is involved in multiple processes including receptor-mediated endocytosis, insulin receptor internalization and glucose transporter type 4 (Glut4) trafficking in adipocytes. RAP6 is also essential for epidermal growth factor receptor (EGFR) trafficking and degradation and phagosome maturation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

GTPase-activating protein and VPS9 domain-containing protein 1 (GAPVD1) also called Rab5-activating protein 6 (RAP6) or Gapex-5 gene is mapped to the human chromosome 9q33.3. It has a carboxy-terminal Vps9-like domain and an amino-terminal Ras GTPase-accelerating proteins (GAP)-like domain. GAPVD1 is present in the plasma membrane and endosomes.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Anti-Gapex-5 recognizes human Gapex-5.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.