Anti-Mlkl

Not available outside of the UK & Ireland.

Application

Anti-MLKL antibody produced in mouse has been used in western blotting.

Biochem/physiol Actions

MLKL activation and subsequent N-terminal 4HB domain unleashing leads to oligomerization and cell membrane entry. This in turn leads to necroptosis.

MLKL (mixed lineage kinase domain-like protein) primarily causes receptor-interacting protein (RIP) kinase–dependent necroptosis. However, during hepatitis, it results in programmed hepatocellular necrosis which is independent of RIPK3. MLKL also participates in endosomal trafficking and the formation of extracellular vesicle.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

MLKL has a C-terminal pseudokinase (PsK) domain and an N-terminal 4-helix bundle (4HB) domain. Both these domains are held together by two brace helices.

The gene MLKL (mixed lineage kinase domain-like protein) is mapped to human chromosome 16q23. Activated MLKL is present at the cell membrane.

Immunogen

MLKL (NP_689862.1, 1 a.a. ~ 471 a.a) full-length human protein.SequenceMENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQYLPPKCMQEIPQEQIKEIKKEQLSGSPWILLRENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKEIKTMKKFESPNILRIFGICIDETVTPPQFSIVMEYCELGTLRELLDREKDLTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQVKLAGFELRKTQTSMSLGTTREKTDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK

Physical form

Solution in phosphate buffered saline, pH 7.4