Anti-CD226/DNAM-1;clone NewE1(AzideFree)

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

This Anti-CD226/DNAM-1 Antibody, clone NewE1 (Azide Free) is validated for use in Western Blotting and Immunoprecipitation and Activity Assay for the detection of CD226/DNAM-1.

Activity Assay Analysis: A representative lot detected CD226/DNAM-1 which induces platelet activation and aggregation (Sherrington, P.D., et al. (1997). J. Biol. Chem. 272:21735-21744).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD226/DNAM-1 in isolated surface proteins from Jurkat and platelets (Sherrington, P.D., et al. (1997). J. Biol. Chem. 272:21735-21744).
MABT341 works by western blotting under both non-reducing and reducing conditions.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CD226/DNAM1 is a T cell expressed plasma membrane receptor protein that plays multiple roles in lymphocyte signaling, intercellular adhesion, lymphokine secretion and cytotoxic T-cell and NK cell mediated killing. CD226 activates natural killer (NK) cell-mediated cytotoxicity and is a key player in the immune synapse between killer T cells and targets. Also, because it recognizes CD155 and nectins CD226/DNAM1 is being examined as a potential anti-cancer agent because soluble CD226 directly inhibits tumor growth by binding CD155 expressed on tumor cells and down regulating proliferation. CD226/DNAM-1 is expressed on Th1, but not Th2 or Th0 cells, it is also expressed on NK cells, CD8+ T-cells, but not developing T cells. CD226/DNAM-1 is also expressed on monocytes and platelets.

Immunogen

Recombinant protein corresponding to human CD226/DNAM-1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Format: Purified

Quality

Evaluated by Western Blotting in CD226 Transfected CHO cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected CD226/DNAM-1 in 10 µg of CD226 Transfected CHO cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~39 kDa observed