Anti-phospho-MAP Kinase Kinase 4 (MKK4, SEK, JNKK1) (pThr223) antibody produced in rabbit, affinity isolated antibody, buffered aqueous glycerol solution

Not available outside of the UK & Ireland.

Application

Anti-phospho-MAP Kinase Kinase 4 may be used for immunoblotting at a working dilution of 1:2,000 using sodium chloride and vanadate-treated 293 cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

SAPK/ERK-1 kinase (SEK1) also called MKK4 or Jun kinase kinase (JNKK) activates the stress activated protein kinase (SAPK). There are other mechanisms by which SAPK may be activated but SEK1 being the immediate upstream activator defines the ensuing protein kinase cascade by the c-Jun transcription factor. Activation of SEK1 occurs through phosphorylation of serine and threonine residues at position 219 and 223 respectively by MEKK. High levels of JNK/SAPK activity are reported to induce apoptosis by the activation of c-Jun. Anti-phospho-MAP Kinase Kinase 4 (MKK4, SEK1, JNKK1 (phosphothreonine 223) detects SEK1/MKK4 protein when activated by phosphorylation at Thr223.

Immunogen

synthetic phospho-Thr²²³ peptide corresponding to residues around Thr²²³ of human SEK1.

Physical form

Solution in 10 mM HEPES, pH 7.5, containing 150 mM sodium chloride, 100 µg/mL bovine serum albumin and 50% glycerol.

Specificity

Does not react with non-phosphorylated MKK4/SEK1 or phosphorylated MKK3, MEK-1 or MEK-2.